Two-, three-, and four-dimensional NMR methods for obtaining larger and more precise three-dimensional structures of proteins in solution.

CONTENTS PE~S~’ECTIVES AND OVERVIEW ........................................................................................ 30 PRINCIPLES OF 3DaND 4D NM~ ...................................................................................... 31 S~Q~ ~SS~G~ ~S~G ~O~UC~ 3~ ~ .............................................. 34 Assignment of Spin Systems ..................................................................................... 34 Conventional Sequential Assi9nment Usin9 3D NMR ............................................... 42 Sequential Assi#nment Via Well-Resolved One-Bond J Couplings ............................. 42